Genetic material, located within the nucleus of a cell, is not randomly organized. Although the spatial configuration of DNA and DNA-associated proteins is known to influence gene expression and cellular function, how this occurs is currently unknown. The Common Fund’s 4D Nucleome program aims to understand the principles behind the three-dimensional organization of the nucleus in space and time (the 4th dimension), the role nuclear organization plays in gene expression and cellular function, and how changes in the nuclear organization affect normal development as well as various diseases.
3D mapping strategy reveals framework for gene expression
Toward understanding 3D genome architecture and its relationship to gene regulation, 4D Nucleome researcher Dr. Yijuan Ruan, Ph.D and his team of international collaborators have identified a framework in which genes are organized and transcribed at the chromosomal level.
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NEW! Seeing transcription in a new light
In a recent article titled "Imaging Transcription: Past, Present, and Future" 4D Nucleome investigator Dr. Robert H Singer and collaborators provide an overview of the evolution and current state of transcription imaging technologies. Also discussed are important concepts and possible future developments that might solve long standing questions in transcriptional regulation and gene expression.
"Unraveling nuclear architecture" named a 2016 Method to Watch
The mapping of nuclear architecture was named a Nature Methods 2016 Method to Watch, with special mention of the 4D Nucleome program.
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4DN Program Awardees Announced
Approximately $25 million has been awarded to 29 research teams to support the development of new mapping and imaging tools, novel computational tools, pilot mapping of the 3D organization of mammalian genomes, and studies to understand and manipulate nuclear architecture. Learn more about these awards here.
Read the press release announcing the launch of 4D Nucleome and other Common Fund programs designed to take aim at gaps in biomedical research.
In the laboratories of Dr. Charles Patrick Lusk and Dr. Joerg Bewersdorf at Yale School of Medicine, Jordan Myers is working with colleagues on the development of a light microscopy technique to visualize nuclear pore complexes. Read the full story here.
Contact us at: 4DNucleome@mail.nih.gov