The Common Fund’s Protein Capture Reagents program is developing new resources and tools to understand the critical role the multitude of cellular proteins play in development, health, and disease. These resources will support a wide-range of research and clinical applications by enabling the isolation and tracking of proteins of interest.
Standardizing Antibody Quality
Antibodies are a crucial component in multiple cell biology applications, but there are currently no standardized methods to assess antibody quality. Researchers, including awardee Anthony Kossiakoff, developed a standard operating procedure (SOP) using mass spectrometry to quantitatively assess antibodies used in immunoprecipitation experiments (IP-MS), representing a major step toward standardizing antibody quality.
The Need for Standardized Antibodies in Research
Two op-ed pieces in Nature and Nature Methods discuss the importance of standardizing antibodies by improving antibody quality and performance in order to enhance reproducibility in biomedical research.
- Reproducibility: Standardize Antibodies Used in Research. Bradbury A and Plückthun A. Nature. 2015 Feb 5; 518(7537):27-29.
- Protein Binder Woes. Nature Methods. 2015 Apr 29; 12(5):373.
Using DNA for More than Genetic Information
Tiny chains of DNA molecules that fold up into variety of 3-dimensional shapes that recognize and bind to a whole host of biological molecules are called aptamers. Protein Capture Reagents Program grantee Dr. H. Tom Soh and colleagues developed a way to make apatmers quickly and have used aptamers in a new device to track the drug levels in an animal’s bloodstream in real time.
Learn more about apatmers here.
Image source: From Ferguson et al. Sci Transl Med 5, 213ra165 (2013). Reprinted with permission from AAAS.
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Schematic of Protein Capture Reagents Program Components
Find information on available affinity reagents for human transcription factors generated by the Protein Capture Reagents Program, including antigen source, binder type, performed validations, and links to distributors.
Protein Capture Reagents Validation - Solicitation Announcement
The National Heart, Lung, and Blood Institute (NHLBI), on behalf of the NIH Common Fund Office, the National Human Genomic Research Institute (NHGRI) and the National Institute on Deafness and Other Communication Disorders (NIDCD), is soliciting proposals under full and open competition procedures to conduct an independent, third party assessment of protein affinity reagents produced by the NIH Common Fund's Protein Capture Reagents Program (PCRP). Learn more about this announcement here.
In the News
CDI Laboratories Showcased at White House Demo Day
The biotech startup CDI Laboratories , whose system of monoclonal antibody production is part of the Protein Capture Reagents program, was showcased at the first-ever White House Demo Day held on August 4, 2015. Ignacio Pino, president of CDI Laboratories, was invited to the White House to represent his company, which produces research-grade protein and antibody products. Pino founded the company in 2008, with Heng Zhu, Seth Blackshaw, Jef Boeke, Dan Eichinger and Joe Bonaventura.