Building Blocks, Biological Pathways, and Networks (BBPN)
Building Blocks, Biological Pathways, and Networks (BBPN)
Laboratory Methods
The Building Blocks, Biological Pathways and Networks program has transitioned from Common Fund support. For more information, please visit https://commonfund.nih.gov/bbpn/index. Please note that since the Building Blocks, Biological Pathways and Networks program is no longer supported by the Common Fund, the program website is being maintained as an archive and will not be updated on a regular basis.
BOEKE Protocols
- Yeast high efficiency transformation - dSLAM (PDF)
- Yeast sporulation - dSLAM (PDF)
COLE Protocols
- Chemical synthesis of a novel coenzyme A-based affinity probe (PDF)
COTTER Protocols
- Quantification of lysine acetylation (PDF)
- N-terminal derivatization for de novo sequencing (PDF)
- Determination of ubiquitylation sites (PDF)
- Determination of SUMOylation sites (PDF)
Frommer Protocols
MBIC Protocals
- Preparing stock solutions of Malachite Green derivatives
MELUH Protocols
- Indirect Immunofluorescence for Yeast (PDF)
- DAPI Staining Yeast (PDF)
- Chromatin Immunoprecipitation (ChIP) for Yeast (PDF)
PANDEY Protocols
- 18O - labeling trypsin digest protocol for relative quantitation by mass spectrometry
RANDO Protocols
- Mononucleosome Chromatin Immunoprecipitation for Yeast (PDF)
NCDIR Protocols
Disruption of Yeast or Bacteria Cells
Subcellular Fractionation
- Yeast NE & NPC Preps
- Yeast Nuclei & SPB Preps
- Carbonate Extraction of NEs
- Rapid Subcell Fractionatn for Nucl & NEs
- Heparin Extraction of NEs
Isolation of Protein Complexes
- Conjugation of Tosylactivated Dynabeads
- Conjugation of Epoxyactivated Dynabeads
- Pullouts with IgG Resin
- Immunoaffinity purifications of protein complexes
Mass Spectrometry
- In-gel Digestion protocol
Genomic Analyses
Protein Biochemistry
