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Laboratory Methods

Looking for information on NIH CRM cell lines? Please visit http://commonfund.nih.gov/stemcells/lines.

Embryonic Stem Cells/Induced Pluripotent Stem Cells

Anti-SeV staining protocol
CD34+ cell reprogramming using episomal vectors
Conditioning pluripotent stem cell media with mouse embryonic fibroblasts (MEF-CM)
Cryopreservation of human pluripotent stem cells in defined conditions
Dispase splitting protocol
FACs staining for intracellular protein
FACs surface staining
Formation of embryoid bodies from matrigel dots protocol
General spinfection protocol
Human fibroblast reprogramming: Lentiviral method (hSTEMCCA)
Immunostaining
iPSC derivation from fibroblast in chemically defined medium
iPSC reprogramming from human peripheral blood using Sendai virus mediated gene transfer
Reprogramming fibroblasts with Sendai
Reprogramming human fibroblasts using an episomal vector system
Retrovirus transfection protocol
Splitting hESC/hiPSC lines on MEF using Accutase
Splitting hESC/hiPSC lines with EDTA in feeder free conditions
Spitting hPSCs with Dispase 
 

Neural Stem Cells

Cryopreserving neural stem cells
Derivation of neural crest cells from human pluripotent stem cells
Differentiating EBs (Rosette formation) and midbrain specification
Differentiating neural stem cells into astrocytes
Differentiating neural stem cells into neurons
Differentiating neural stem cells into oligodendrocytes
Dopaminergic neuron differentiation
Isolating dopaminergic progenitors
Midbrain dopamine neurons from hESCs
Neural induction - dual SMAD inhibition
Notes of markers for neural differentiation
Passaging neural stem cells (adherent culture)
Passaging neural stem cells (suspension culture) 
 

Hematopoietic Stem Cells

Hematopoietic differentiation
Mesoderm differentiation hematopoiesis 
 

Mesenchymal Stem Cells

Cardiac differentiation
Chondrocyte protocol 
 

Others

Pancreatic differentiation
Protocol for directed differentiation of human pluripotent stem cells toward a hepatocyte fate
Robust generation of hepatocyte-like cells from human embryonic stem cell populations

 

 

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