Program Snapshot

 

The Single Cell Analysis Program (SCAP) has transitioned from Common Fund support. Common Fund programs are strategic investments that achieve a set of high-impact goals within a 5-10 year timeframe. At the conclusion of each program, deliverables will transition to other sources of support or use within the scientific community. SCAP was supported by the Common Fund from 2012 to 2017. SCAP's major accomplishments include new and unique tools, which are enabling researchers to carry out experiments that were not previously possible. Examples include transcriptome in vivo analysis (TIVA) tag for RNA capture, inDrop for high throughput single cell transcriptomics, fluorescent in situ sequencing, whole genome nuclear sequencing and the dual-view inverted selective plane illumination microscope (diSPIM) imaging system, which provides tracking of cell lineage in living, multicellular organisms - to name a few. TSCAP has also significantly moved the field of single molecule fluorescent in situ hybridization for RNA and protein detection forward, including the detection of single nucleotide variants, multiplexing the detection of tens of RNAs and proteins and RNA detection into live cells using CRISPR. A further consequence of SCAP is the establishment of the SCAP-Transcriptome Consortium project, which developed a public portal with phenotypic information and next generation sequencing data of whole transcriptome for 697 single cells from 56 subjects. The SCAP-T Consortium has also established procedures for depositing meta-data and RNAseq data to the NIH National Center for Biotechnology Information (NCBI) database of Genotypes and-Phenotype (dbGaP).

Please note that since the SCAP program is no longer supported by the Common Fund, the program website is being maintained as an archive and will not be updated on a regular basis. 

 

This page last reviewed on October 13, 2017