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Stem Cell Lines

All available lines are currently distributed through RUCDR Infinite Biologics  at Rutgers University. Please visit www.stemcells.nindsgenetics.org/  to request clinical grade iPSC lines or www.nimhgenetics.org/stem_cells/crm_lines.php to request research grade iPSC lines

Looking for information on RMP methods and protocols? Please visit http://commonfund.nih.gov/stemcells/methods.

Frequently asked questions for requesting stem cell lines made by the Regenerative Medicine Program
 

 
RMP (phase I) Stem Cell Lines
Cell Line Description Type Parental Line/Starting Material iPSC Reprogramming Method Status
LiPSC-GR1.1 Current good manufacturing practices line (male) Clinical Grade CD34+ cord blood Episomal plasmid Available
LiPSC-GR1.1 non-Current good manufacturing practices line (male) Research Grade CD34+ cord blood Episomal plasmid Available
NCRM-1 NIH CRM control iPSC line (male) Control reference line CD34+ cord blood Episomal plasmid Available
NCRM-2 NIH CRM control iPSC line (female) Control reference line CD34+ cord blood Episomal plasmid Available
NCRM-3 NIH CRM control iPSC line (male) Control reference line CD34+ cord blood Episomal plasmid Available
NCRM-4 NIH CRM control iPSC line (female) Control reference line CD34+ cord blood Episomal plasmid Available
NCRM-5 NIH CRM control iPSC line (male) Control reference line CD34+ cord blood Episomal plasmid Available
NCRM-6 NIH CRM control iPSC line (female) Control reference line CD34+ cord blood Episomal plasmid Available
ND1.4 NIH CRM control iPSC line Control reference line Fibroblast (ATCC) Episomal plasmid Available
ND2.0 NIH CRM control iPSC line Control reference line Fibroblast (ATCC) Episomal plasmid Available
CY2 NIH CRM control iPSC line Control reference line Blood Episomal plasmid Available
NCRM5-AS1-iCLHN NCRM-5 iPSCs targeted with NanoLuc-HaloTag at AAVS1 safe harbor Luciferase reporter line NCRM-5 Episomal plasmid Available
NCRM5-C13-iCLHN NCRM-5 iPSCs targeted with NanoLuc-HaloTag at Chr. 13 safe harbor Luciferase reporter line NCRM-5 Episomal plasmid Available
NCRM5-AS1-iCAGcGFP NCRM-5 iPSCs targeted with copGFP at AAVS1 safe harbor GFP reporter line NCRM-5 Episomal plasmid Available
T21C1 iPSC line from Down's syndrome patient (Trisomy 21) Trisomy 21 Down Syndrome line Patient fibroblasts (from Coriell) Retroviral Available
T21C5 iPSC line from Down's syndrome patient in which trisomy 21 karyotype reverted to normal Trisomy 21 Down Syndrome line Patient fibroblasts (from Coriell) Retroviral Available

Publications that used RMP stem cell lines

  1. Baghbaderani BA, Syama A, Sivapatham R, Pei Y, Mukherjee O, Fellner T, Zeng, Rao MS. Detailed Characterization of Human Induced Pluripotent Stem Cells Manufactured for Therapeutic Applications. Stem Cell Rev. 2016 Jun 10.
  2. Baghbaderani BA, Tian X, Neo BH, Burkall A, Dimezzo T, Sierra G, Zeng X, Warren K, Kovarcik DP, Fellner T, Rao MS. cGMP-Manufactured Human Induced Pluripotent Stem Cells Are Available for Pre-clinical and Clinical Applications. Stem Cell Reports. 5:647-659, 2015.
  3. Xue H, Wu J, Li S, Rao MS, Liu Y. Genetic Modification in Human Pluripotent Stem Cells by Homologous Recombination and CRISPR/Cas9 System. Methods Mol Biol. 1307:173-90, 2016.
  4. Efthymiou AG, Steiner J, Pavan WJ, Wincovitch S, Larson DM, Porter FD, Rao MS, Malik N. Rescue of an in vitro neuron phenotype identified in Niemann-Pick disease, type C1 induced pluripotent stem cell-derived neurons by modulating the WNT pathway and calcium signaling. Stem Cells Transl Med. 4:230-8, 2015.
  5. Li S, Xue H, Wu J, Rao MS, Kim DH, Deng W, Liu Y. Human Induced Pluripotent Stem Cell NEUROG2 Dual Knockin Reporter Lines Generated by the CRISPR/Cas9 System. Stem Cells Dev. 24:2925-42, 2015.
  6. Pei Y, Sierra G, Sivapatham R, Swistowski A, Rao MS, Zeng X. A platform for rapid generation of single and multiplexed reporters in human iPSC lines. Sci Rep. 5:9205, 2015.
  7. Cerbini T, Luo Y, Rao MS, Zou J. Transfection, selection, and colony-picking of human induced pluripotent stem cells TALEN-targeted with a GFP gene into the AAVS1 safe harbor. J Vis Exp. (96), 2015.
  8. Cerbini T, Funahashi R, Luo Y, Liu C, Park K, Rao M, Malik N, Zou J. Transcription activator-like effector nuclease (TALEN)-mediated CLYBL targeting enables enhanced transgene expression and one-step generation of dual reporter human induced pluripotent stem cell (iPSC) and neural stem cell (NSC) lines. PLoS One. 10:e0116032, 2015
  9. Luo Y, Liu C, Cerbini T, San H, Lin Y, Chen G, Rao MS, Zou J. Stable enhanced green fluorescent protein expression after differentiation and transplantation of reporter human induced pluripotent stem cells generated by AAVS1 transcription activator-like effector nucleases. Stem Cells Transl Med. 3:821-35, 2015.
  10. Malik N, Efthymiou AG, Mather K, Chester N, Wang X, Nath A, Rao MS, Steiner JP. Compounds with species and cell type specific toxicity identified in a 2000 compound drug screen of neural stem cells and rat mixed cortical neurons. Neurotoxicology. 45:192-200, 2014.
  11. Efthymiou A, Shaltouki A, Steiner JP, Jha B, Heman-Ackah SM, Swistowski A, Zeng X, Rao MS, Malik N. Functional screening assays with neurons generated from pluripotent stem cell-derived neural stem cells. J Biomol Screen. 19:32-43, 2014.
  12. Malik N, Wang X, Shah S, Efthymiou AG, Yan B, Heman-Ackah S, Zhan M, Rao M. Comparison of the gene expression profiles of human fetal cortical astrocytes with pluripotent stem cell derived neural stem cells identifies human astrocyte markers and signaling pathways and transcription factors active in human astrocytes. PLoS One. 9:e96139, 2014.
  13. Ou W, Li P, Reiser J. Targeting of herpes simplex virus 1 thymidine kinase gene sequences into the OCT4 locus of human induced pluripotent stem cells. PLoS One. 8:e81131, 2013.
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