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Laboratory Methods

The Epigenomics program has transitioned from Common Fund support. For more information, please visit https://commonfund.nih.gov/epigenomics.

Please note that since the Epigenomics program is no longer supported by the Common Fund, the program website is being maintained as an archive and will not be updated on a regular basis.

The Common Fund supported Epigenomics program produced reference epigenomes of a wide-range of normal cell types that can be used by the community for a variety of purposes. This was made possible through the development of protocols that leveraged advancements in next-generation sequencing technologies to map DNA methylation, histone modifications, chromatin accessibility, and small RNA transcripts in stem cells and primary ex vivo tissues. The Roadmap Epigenomics Consortium (link is external) also made these protocols as well as data standards available and they can be downloaded here.

Experimental Protocols

Methylome Analysis

DNase1

General Protocols

ChIP-Seq

RNA Analysis

Data Standards

Methylome Analysis

ChIP-Seq

RNA Analysis

This page last reviewed on January 30, 2024