Laboratory Methods

• Yeast high efficiency transformation - dSLAM (PDF)
• Yeast sporulation - dSLAM (PDF)

• Chemical synthesis of a novel coenzyme A-based affinity probe (PDF)

• Quantification of lysine acetylation (PDF)
• N-terminal derivatization for de novo sequencing (PDF)
• Determination of ubiquitylation sites (PDF)
• Determination of SUMOylation sites (PDF)

• Protocol for monitoring intracellular metabolite levels

• Preparing stock solutions of Malachite Green derivatives

• Indirect Immunofluorescence for Yeast (PDF)
• DAPI Staining Yeast (PDF)
• Chromatin Immunoprecipitation (ChIP) for Yeast (PDF)

• 18O - labeling trypsin digest protocol for relative quantitation by mass spectrometry
• SILAC: Stable Isotope labeling by amino acids in cell culture

• Mononucleosome Chromatin Immunoprecipitation for Yeast (PDF)

• Harvesting Cells and Making Noodles
• Cryogenic Disruption Using PM100 Ball Mill
• Cryogenice Disruption of Yeast Cells PM100
• Microfluidizer

• Yeast NE & NPC Preps
• Yeast Nuclei & SPB Preps
• Carbonate Extraction of NEs
• Rapid Subcell Fractionatn for Nucl & NEs
• Heparin Extraction of NEs

• Conjugation of Tosylactivated Dynabeads
• Conjugation of Epoxyactivated Dynabeads
• Pullouts with IgG Resin
• Immunoaffinity purifications of protein complexes

• In-gel Digestion protocol

• mRNA expression microarrays

• Carbonate Silver Stain
  
• Electropurification
• Methanol Precipitation of Protein
• Protease Mapping
• Strip Blots
• Whole Cell Lysis and TCA Precipitation