Transformative R01 Program

CELL-BASED SCREENING AND SELECTION OF CYCLOTIDE-BASED CAPTURE REAGENTS FOR PROTEIN PROFILING USING MICRO-ARRAY FORMAT

CELL-BASED SCREENING AND SELECTION OF CYCLOTIDE-BASED CAPTURE REAGENTS FOR PROTEIN PROFILING USING MICRO-ARRAY FORMAT

The use of cell-based libraries of cyclotides for selecting specific cyclotide sequences against particular protein targets. Cyclotides are a new emerging family of plant-derived backbone-cyclized polypeptides (≈28-37 amino acids long) that share a disulfide-stabilized core (3 disulfide bonds) characterized by an unusual knotted structure. Their unique circular backbone topology and knotted arrangement of three disulfide bonds makes them exceptionally stable to thermal and enzymatic degradation.