Transformative R01 Program

DEVELOPMENT OF HIGH THROUGHPUT APTAMER-BASED PROTEIN CAPTURE/DETECTION ASSAYS TOWARDS COMPREHENSIVE ANALYSIS OF HUMAN PROTEOME

DEVELOPMENT OF HIGH THROUGHPUT APTAMER-BASED PROTEIN CAPTURE/DETECTION ASSAYS TOWARDS COMPREHENSIVE ANALYSIS OF HUMAN PROTEOME

​​The schematics of multiplexed microfluidic SELEX. Aptamers in a randomized nucleic acid (NA) pool will bind target proteins confined in sol-gel spots on the microfluidic device, protein-bound aptamers will be thermally eluted by integrated aluminum electrodes. Then, selected aptamers are either reamplified and used for the next round of SELEX or identified by massively parallel sequencing.