Topic 2

2/24/2012 8:50:38 AM #

We need to develop the equivalent of next-gen/deep sequencing technologies for proteins that are having such a high impact with DNA and RNA. Moreover, instead of simply catching up to genomics, we might think of in situ protein sequencing to monitor proteins and their interactions in a crowded cellular context. Single molecule imaging, on a massively parralel manner, comes to mind.

Andrew Emili | Reply

3/2/2012 8:48:10 AM #

Currently, non-MS based method such as multiplex array  can provide quantitative data  in a broad  dynamic range without any separation or extraction steps.  The technology platform needs to be evaluated further to determine its  pros and cons.  In addition, new platform of multiplex method should be promoted.  It is time to focus on proteins-the key element for functioning.

Anonymous | Reply

3/7/2012 7:42:30 AM #

The clue is in the title call "Disruptive", not incremental. A proteomics or Biomarker Discovery & Validation Platform should have the following characteristics at a minimum :
- simple, easy to use instrumentation and bioinformatics
- able to rapidly detect, identify and quantify all human proteins
- rapidly detect & quantify virtually all proteins from any organism
- discover previously unknown proteins
- detect post-translational modifications
- with the ultimate in high sensitivity detection
A whole human proteome screen within a matter of days (cf. DNA NGS systems) is certainly achievable it just requires a little lateral thinking.

Stephen Osborne @PastelBio | Reply